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Article

Relationship of vector insert size to homologous integration during transformation of Neurospora crassa with the cloned am (GDH) gene.

Molecular & general genetics : MGG - 1 Mar 1990

Asch D K, Kinsey J A

Abstract excerpt

We used lambda and plasmid vectors containing the am+ gene in an insert of from 2.7 to 9.1 kb, to transform am point mutant and deletion strains. A total of 199 transformants were examined with the potential to yield am+ transformants by homologous recombination. When we used vectors that had 9.1 kb of homology with the chromosomal DNA, 30% of the transformants obtained were the result of homologous recombination...

Topics

Bacteriophage lambda
Cloning, Molecular
DNA Transposable Elements
DNA, Fungal
DNA, Recombinant
Genetic Vectors
Glutamate Dehydrogenase
Mutation
Neurospora
Neurospora crassa
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sequence Homology, Nucleic Acid

Transformation, Genetic

Source

Identifiers

PMID: 2157957
DOI: 10.1007/BF00280365

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2157957-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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