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Article

Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585.

Applied microbiology and biotechnology - 1 Feb 1990

Piret J, Resendiz B, Mahro B, Zhang J Y, Serpe E, Romero J, Connors N, Demain A L

Abstract excerpt

We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities...

Topics

Bacterial Proteins
Cephalosporins
Cloning, Molecular
DNA, Bacterial
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Mutation
Oxidoreductases
Peptide Synthases
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Plasmids

Restriction Mapping

Streptomyces

Transformation, Bacterial

Source

Identifiers

PMID: 1366440
DOI: 10.1007/BF00173728

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1366440-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585.PMID 1366440Read ↗

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