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Article

Characterization and complementation of a cephalosporin-deficient mutant of Streptomyces clavuligerus NRRL 3585.

Applied microbiology and biotechnology - 1 Feb 1990

Piret J, Resendiz B, Mahro B, Zhang J Y, Serpe E, Romero J, Connors N, Demain A L

Abstract excerpt

We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region was cloned which partially restores antibiotic production, ACV synthetase and cyclase activities...

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PMID: 1366440
DOI: 10.1007/BF00173728

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