Characterization of the Streptomyces clavuligerus argC gene encoding N-acetylglutamyl-phosphate reductase: expression in Streptomyces lividans and effect on clavulanic acid production.

Abstract excerpt

The argC gene of Streptomyces clavuligerus encoding N-acetylglutamyl-phosphate reductase (AGPR) has been cloned by complementation of argC mutants Streptomyces lividans 1674 and Escherichia coli XC33. The gene is contained in an open reading frame of 1,023 nucleotides which encodes a protein of 340 amino acids with a deduced molecular mass of 35,224 Da. The argC gene is linked to argE, as shown by complementation...

Topics

• Aldehyde Oxidoreductases
• Amino Acid Sequence
• Anti-Bacterial Agents
• Base Sequence
• Clavulanic Acid
• Clavulanic Acids
• Cloning, Molecular
• Genetic Complementation Test
• Molecular Sequence Data
• Mutation