Article
Isolation of peroxisome assembly mutants from Saccharomyces cerevisiae with different morphologies using a novel positive selection procedure.
The Journal of cell biology - 1 Oct 1992
Van der Leij I, Van den Berg M, Boot R, Franse M, Distel B, Tabak H F
Abstract excerpt
We have developed a positive selection system for the isolation of Saccharomyces cerevisiae mutants with disturbed peroxisomal functions. The selection is based on the lethality of hydrogen peroxide (H2O2) that is produced in wild type cells during the peroxisomal beta-oxidation of fatty acids. I...
Topics
- Acetyl-CoA C-Acetyltransferase
- Blotting, Western
- Catalase
- Electron Transport Complex IV
- Hydrogen Peroxide
- Microbodies
- Microscopy, Immunoelectron
- Mutagenesis
- Phenotype
- Saccharomyces cerevisiae
- Selection, Genetic