Article
Radical effects on mutation spectra in lambda phage.
International journal of radiation biology - 1 Jul 1992
Bertram H, Hagen U
Abstract excerpt
Mutations in the lambda repressor gene cI (710 bp) were induced by 60Co-gamma radiation in dissolved lambda phage DNA. After in vitro DNA packaging to lambda phage particles (pack phage) and phenotypic expression of the mutants, DNA was sequenced directly. Two-thirds of mutations were located in the amino terminus region of the gene without any signs of hotspots. Changes consisted of (+1) insertions (25%) and...
Topics
- Bacteriophage lambda
- Base Sequence
- Cobalt Radioisotopes
- DNA, Viral
- Gamma Rays
- Molecular Sequence Data
- Mutation