Article
Role of Pseudomonas aeruginosa outer membrane protein OprH in polymyxin and gentamicin resistance: isolation of an OprH-deficient mutant by gene replacement techniques.
Antimicrobial agents and chemotherapy - 1 Nov 1992
Young M L, Bains M, Bell A, Hancock R E
Abstract excerpt
The cloned oprH gene of Pseudomonas aeruginosa was mutated by inserting a 1.4-kbp fragment that encodes tetracycline resistance. This mutated gene was then incorporated into the P. aeruginosa chromosome by homologous recombination. Growth of the resultant oprH::tet mutant in Mg(2+)-deficient medium had little effect on susceptibility to polymyxin B, gentamicin, or EDTA unless the mutant was complemented by the...
Topics
- Bacterial Outer Membrane Proteins
- Cloning, Molecular
- Drug Resistance, Microbial
- Edetic Acid
- Gene Expression
- Genes, Bacterial
- Gentamicins
- Magnesium
- Magnesium Deficiency
- Mutagenesis, Insertional