Article
Cyclin A-Cys41 does not undergo cell cycle-dependent degradation in Xenopus extracts.
FEBS letters - 13 Jul 1992
Lorca T, Devault A, Colas P, Van Loon A, Fesquet D, Lazaro J B, Dorée M
Abstract excerpt
Truncated cyclin A and cyclin B lacking the N-terminal domain comprising the 'destruction box' escape from proteolysis and arrest cells at metaphase. Mutation of a conserved arginine residue of the destruction domain makes cyclin B resistant to proteolysis. Here we show that mutation of the same residue also makes cyclin A resistant to proteolysis, in either of two situations in which the cyclin degradation...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Animals
- Blotting, Western
- Cations, Divalent
- Cell Cycle
- Cyclins
- Cysteine
- Magnesium
- Molecular Sequence Data
- Mutation
- Protamine Kinase