Immortalization of human aortic smooth muscle cells with origin-minus simian virus 40 DNA.

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Article

Immortalization of human aortic smooth muscle cells with origin-minus simian virus 40 DNA.

Biotechnology and applied biochemistry - 1 Oct 1992

Murahashi N, Sasaguri Y, Ohuchida M, Kakita N, Morimatsu M

Abstract excerpt

We established two cell lines of human smooth muscle cells (SMC) by transfection of cells from the aortic intima and aortic media with origin-minus simian virus 40 (ori-minus SV40) DNA. Ori-minus SV40 DNA very efficiently immortalized human smooth muscle cells in culture. Proteins that these cell lines produced included type I, III, IV, and V collagens, fibronectin, and human matrix metalloproteinases (MMP)-1...

Topics

Animals
Antigens, Polyomavirus Transforming
Aorta
Cell Line, Transformed
Collagen
Collagenases
DNA, Viral
Fibronectins
Humans
Matrix Metalloproteinase 1
Matrix Metalloproteinase 3

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Metalloendopeptidases

Mice

Mice, Inbred BALB C

Mice, Nude

Muscle, Smooth, Vascular

Neoplasms, Experimental

Phenotype

Source

Open in PubMed

Identifiers

PMID: 1333771

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1333771-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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