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Article

A cell-free recombination system for site-specific integration of multigenic shuttle plasmids into the herpes simplex virus type 1 genome.

Journal of virology - 1 Sept 1992

Gage P J, Sauer B, Levine M, Glorioso J C

Abstract excerpt

This report describes a novel method for complementation studies of defective herpes simplex virus (HSV) genes. Viral test gene and nonviral reporter gene cassettes were rapidly integrated into the HSV genome in a site-specific and reversible manner by using the P1 phage-based Cre-lox recombinati...

Topics

Animals
Cell-Free System
Genetic Complementation Test
Genetic Vectors
Genome, Viral
Mutagenesis, Insertional
Phenotype
Plasmids
Recombinant Fusion Proteins
Recombination, Genetic
Simplexvirus
Vero Cells

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Identifiers

PMID: 1323709
DOI: 10.1128/JVI.66.9.5509-5515.1992
PMCID: PMC289109

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1323709-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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