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Article

A cell-free recombination system for site-specific integration of multigenic shuttle plasmids into the herpes simplex virus type 1 genome.

Journal of virology - 1 Sept 1992

Gage P J, Sauer B, Levine M, Glorioso J C

Abstract excerpt

This report describes a novel method for complementation studies of defective herpes simplex virus (HSV) genes. Viral test gene and nonviral reporter gene cassettes were rapidly integrated into the HSV genome in a site-specific and reversible manner by using the P1 phage-based Cre-lox recombination system. Shuttle plasmids contained a functional loxP recombination site, an expressible form of the bacterial lacZ...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 1323709
DOI: 10.1128/JVI.66.9.5509-5515.1992
PMCID: PMC289109

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Source receipt: pubmed, retrieved 7 Sept 2026.

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