Article
Selection of variant hepatoma cells in liver-specific growth media: regulation at the mRNA level.
Differentiation; research in biological diversity - 1 May 1992
Armbruster L, Cavard C, Briand P, Bertolotti R
Abstract excerpt
Three liver-specific growth media, respectively free of arginine (Arg-), tyrosine (Tyr-) and glucose (G-), have been used to characterize cells of the rat H4IIEC3, human HepG2 and mouse BW hepatoma lines. Cells of clone FaO, a derivative of line H4IIEC3, freely grew in Tyr- and G- media, and gave rise to stable variants in Arg- conditions. Cells of line HepG2 and clone BWTG3, a derivative of line BW, degenerated...
Topics
- Animals
- Blotting, Northern
- Blotting, Southern
- Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor
- Carcinoma, Hepatocellular
- Clone Cells
- Culture Media
- Fibroblasts
- Genetic Variation
- Humans
- Ligases