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Article

The organization of the Pm promoter of the TOL plasmid reflects the structure of its cognate activator protein XylS.

Molecular & general genetics : MGG - 28 Sept 1994

Kessler B, Timmis K N, de Lorenzo V

Abstract excerpt

The toluate catabolic operon carried by the TOL plasmid pWW0 of Pseudomonas putida is positively regulated by the benzoate-responsive XylS protein which, when activated, stimulates transcription from the operon promoter Pm. In this study, the mode in which XylS effects the activity of the Pm prom...

Topics

Amino Acid Sequence
Bacterial Proteins
Base Sequence
DNA Transposable Elements
DNA-Binding Proteins
Genes, Bacterial
Genetic Vectors
Molecular Sequence Data
Mutagenesis, Site-Directed
Operon
Phenotype
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Promoter Regions, Genetic

Pseudomonas putida

Sequence Deletion

Toluene

Trans-Activators

Source

Identifiers

PMID: 7969028
DOI: 10.1007/BF00282749

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7969028-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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The organization of the Pm promoter of the TOL plasmid reflects the structure of its cognate activator protein XylS.PMID 7969028Read ↗

Mutations leading to constitutive expression from the TOL plasmid meta-cleavage pathway operon are located at the C-terminal end of the positive regulator protein XylS.Semantic proximity Read ↗

Identification of critical amino-terminal regions of XylS. The positive regulator encoded by the TOL plasmid.Semantic proximity Read ↗

Cross talk between catabolic pathways in Pseudomonas putida: XylS-dependent and -independent activation of the TOL meta operon requires the same cis-acting sequences within the Pm promoter.Semantic proximity Read ↗

XylS domain interactions can be deduced from intraallelic dominance in double mutants of Pseudomonas putida.Semantic proximity Read ↗

XylS-Pm promoter interactions through two helix-turn-helix motifs: identifying XylS residues important for DNA binding and activation.Semantic proximity Read ↗

Signal-regulator interactions. Genetic analysis of the effector binding site of xylS, the benzoate-activated positive regulator of Pseudomonas TOL plasmid meta-cleavage pathway operon.Semantic proximity Read ↗

Mutational analysis of the highly conserved C-terminal residues of the XylS protein, a member of the AraC family of transcriptional regulators.Semantic proximity Read ↗

A comparison of the multiple alleles of xylS carried by TOL plasmids pWW53 and pDK1 and its implications for their evolutionary relationship.Semantic proximity Read ↗

A general system to integrate lacZ fusions into the chromosomes of gram-negative eubacteria: regulation of the Pm promoter of the TOL plasmid studied with all controlling elements in monocopy.Semantic proximity Read ↗

Residues 137 and 153 at the N terminus of the XylS protein influence the effector profile of this transcriptional regulator and the sigma factor used by RNA polymerase to stimulate transcription from its cognate promoter.Semantic proximity Read ↗

A T7 RNA polymerase-based system for the construction of Pseudomonas strains with phenotypes dependent on TOL-meta pathway effectors.Semantic proximity Read ↗

Genetic evidence of separate repressor and activator activities of the XylR regulator of the TOL plasmid, pWW0, of Pseudomonas putida.Semantic proximity Read ↗

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