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Article

Variations in the cytomegalovirus major immediate-early gene found by direct genomic sequencing.

Journal of clinical microbiology - 1 Apr 1992

Brytting M, Wahlberg J, Lundeberg J, Wahren B, Uhlén M, Sundqvist V A

Abstract excerpt

An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a colorimetric assay for the detection of immobilized amplified nucleic acids and a standard...

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Identifiers

PMID: 1315340
DOI: 10.1128/jcm.30.4.955-960.1992
PMCID: PMC265193

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