Article
Variations in the cytomegalovirus major immediate-early gene found by direct genomic sequencing.
Journal of clinical microbiology - 1 Apr 1992
Brytting M, Wahlberg J, Lundeberg J, Wahren B, Uhlén M, Sundqvist V A
Abstract excerpt
An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a colorimetric assay for the detection of immobilized amplified nucleic acids and a standard...
Topics
- Amino Acid Sequence
- Base Sequence
- Cytomegalovirus
- DNA, Viral
- Evaluation Studies as Topic
- Genes, Viral
- Genetic Variation
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Sensitivity and Specificity