Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Different Real-Time PCR Formats Compared for the Quantitative Detection of Human Cytomegalovirus DNA

1 Nov 1999

Abstract excerpt

BACKGROUND: The aim of this study was to compare the ABI PRISM 7700 Sequence Detection System and the LightCycler to develop a quantitative real-time PCR assay for the detection of human cytomegalovirus (HCMV) DNA suitable for routine hospital application. METHODS: We used one exonuclease probe and five different hybridization probe sets as sequence-specific fluorescence detection formats. For the exonuclease...

Source

Identifiers

PMID: 10545062
DOI: 10.1093/clinchem/45.11.1932

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Different Real-Time PCR Formats Compared for the Quantitative Detection of Human Cytomegalovirus DNAPMID 10545062Read ↗

Determination of the Biological Form of Human Cytomegalovirus DNA in the Plasma of Solid-Organ Transplant RecipientsSemantic proximity Read ↗

Multicentric evaluation of a new commercial cytomegalovirus real-time PCR quantitation assaySemantic proximity Read ↗

Comparative evaluation of published cytomegalovirus primers for rapid real-time PCR: which are the most sensitive?Semantic proximity Read ↗

Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real-Time PCR AssaySemantic proximity Read ↗

Cytomegalovirus sequence variability, amplicon length, and DNase-sensitive non-encapsidated genomes are obstacles to standardization and commutability of plasma viral load results.Semantic proximity Read ↗

Intrinsic deoxyguanosine quenching of fluorescein-labeled hybridization probes: a simple method for real-time PCR detection and genotyping.Semantic proximity Read ↗

Multiplex, Quantitative, Real-Time PCR Assay for Cytomegalovirus and Human DNASemantic proximity Read ↗

Development of Real-Time PCR Assays for the Quantitative Detection of Epstein-Barr Virus and Cytomegalovirus, Comparison of TaqMan Probes, and Molecular BeaconsSemantic proximity Read ↗

Real-time PCR diagnostics failure caused by nucleotide variability within exon 4 of the human cytomegalovirus major immediate-early gene.Semantic proximity Read ↗

A real-time TaqMan PCR for routine quantitation of cytomegalovirus DNA in crude leukocyte lysates from stem cell transplant patientsSemantic proximity Read ↗

Are We There Yet? Impact of the First International Standard for Cytomegalovirus DNA on the Harmonization of Results Reported on Plasma SamplesSemantic proximity Read ↗

Detecting episomal or integrated human papillomavirus 16 DNA using an exonuclease V-qPCR-based assaySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.