Article
Ubiquitin, a central component of selective cytoplasmic proteolysis, is linked to proteins residing at the locus of non-selective proteolysis, the vacuole.
FEBS letters - 20 Apr 1992
Simeon A, van der Klei I J, Veenhuis M, Wolf D H
Abstract excerpt
Ubiquitin, an evolutionary highly conserved protein, is known to be involved in selective proteolysis in the cytoplasm. Here we show that ubiquitin-protein conjugates are also found in the yeast vacuole. Mutants defective in the major vacuolar endopeptidases, proteinase yscA and yscB, lead to accumulation of ubiquitin-protein conjugates in this cellular organelle.
Topics
- Aspartic Acid Endopeptidases
- Cytoplasm
- Fungal Proteins
- Immunoblotting
- Microscopy, Electron
- Mutation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Serine Endopeptidases
- Ubiquitins
- Vacuoles