Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination.

doi:10.1128/JVI.66.4.1841-1848.1992

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Article

Repair and mutagenesis of the genome of a deletion mutant of the coronavirus mouse hepatitis virus by targeted RNA recombination.

Journal of virology - 1 Apr 1992

Koetzner C A, Parker M M, Ricard C S, Sturman L S, Masters P S

Abstract excerpt

The genetic characterization of a nucleocapsid (N) protein mutant of the coronavirus mouse hepatitis virus (MHV) is described. The mutant, Albany 4 (Alb4), is both temperature sensitive and thermolabile. Analysis of the progeny of a mixed infection showed that the defective Alb4 allele is recessive to wild type, and its gene product is diffusible. The N protein of Alb4 was found to be smaller than its wild-type...

Topics

Amino Acid Sequence
Animals
Base Sequence
Capsid
Cell Line
Chromosome Deletion
DNA Repair
DNA, Viral
Electrophoresis, Polyacrylamide Gel
Genome, Viral
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Murine hepatitis virus

Mutagenesis

Mutation

Polymerase Chain Reaction

RNA, Viral

Recombination, Genetic

Viral Core Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 1312608
DOI: 10.1128/JVI.66.4.1841-1848.1992
PMCID: PMC288970

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1312608-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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