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Article

Optimization of targeted RNA recombination and mapping of a novel nucleocapsid gene mutation in the coronavirus mouse hepatitis virus.

Journal of virology - 1 Jan 1994

Masters P S, Koetzner C A, Kerr C A, Heo Y

Abstract excerpt

We have recently described a method of introducing site-specific mutations into the genome of the coronavirus mouse hepatitis virus (MHV) by RNA recombination between cotransfected genomic RNA and a synthetic subgenomic mRNA (C. A. Koetzner, M. M. Parker, C. S. Ricard, L. S. Sturman, and P. S. Masters, J. Virol. 66:1841-1848, 1992). By using a thermolabile N protein mutant of MHV (Alb4) as the recipient virus and...

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Identifiers

PMID: 8254744
DOI: 10.1128/JVI.68.1.328-337.1994
PMCID: PMC236292

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