Article
Cellular localization of Nef expressed in persistently HIV-1-infected low-producer astrocytes.
AIDS (London, England) - 1 Dec 1992
Kohleisen B, Neumann M, Herrmann R, Brack-Werner R, Krohn K J, Ovod V, Ranki A, Erfle V
Abstract excerpt
OBJECTIVES: The characterization and localization of HIV-1 Nef highly expressed in permanently infected astrocytes (TH4-7-5) as a model for latent infection of human brain cells. DESIGN: Immunochemical methods are an appropriate tool to investigate expression and localization of cellular proteins. METHODS: Nef expression was analysed by Western blot and immunoperoxidase staining using a panel of monoclonal and...
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