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Article

Rearrangement of charge-charge interactions in variant ubiquitins as detected by double-mutant cycles and NMR.

Journal of molecular biology - 26 Sept 2003

Sundd Monica, Robertson Andrew D

Abstract excerpt

Previous studies of ubiquitin disclosed numerous charge-charge interactions on the protein's surface. To investigate how neighboring residues influence the strength of these interactions, double-mutant cycles are combined with pK(a) determinations by 2D NMR. More specifically, the environment around the Asp21-Lys29 ion pair has been altered through mutations at position 25, which is an asparagine in mammalian...

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Identifiers

PMID: 12972262
DOI: 10.1016/s0022-2836(03)00995-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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