Article
Elimination of the C-cap in ubiquitin - structure, dynamics and thermodynamic consequences.
Biophysical chemistry - 1 Mar 2007
Ermolenko Dmitri N, Dangi Bindi, Gvritishvili Anzor, Gronenborn Angela M, Makhatadze George I
Abstract excerpt
Single amino acid substitutions rarely produce substantial changes in protein structure. Here we show that substitution of the C-cap residue in the alpha-helix of ubiquitin with proline (34P variant) leads to dramatic structural changes. The resulting conformational perturbation extends over the last two turns of the alpha-helix and leads to enhanced flexibility for residues 27-37. Thermodynamic analysis of this...
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