Article
RNase ES of Streptomyces coelicolor A3(2) can complement the rne and rng mutations in Escherichia coli.
Bioscience, biotechnology, and biochemistry - 1 Aug 2003
Inagawa Takabumi, Okamoto Susumu, Wachi Masaaki, Ochi Kozo
Abstract excerpt
The Streptomyces coelicolor gene SCC88.10c encodes a protein (RNase ES) which is homologous to endoribonucleases in the RNase E/G family. We expressed S. coelicolor RNase ES as a 6 x His-tagged protein in an Escherichia coli mutant carrying a rng (which encodes RNase G) or a rne (which encodes RNase E) mutation to study whether S. coelicolor RNase ES is able to complement these mutations in host E. coli cells....
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