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Article

Probing the ground state structure of the green fluorescent protein chromophore using Raman spectroscopy.

Biochemistry - 18 Apr 2000

Bell A F, He X, Wachter R M, Tonge P J

Abstract excerpt

We present Raman spectra, obtained using 752 nm excitation, on wild-type GFP and the S65T mutant of this intrinsically fluorescent protein together with data on a model chromophore, ethyl 4-(4-hydroxyphenyl)methylidene-2-methyl-5-oxoimidazolacetate . In the pH range 1-14, the model compound has two macroscopic pK(a)s of 1.8 and 8.2 attributed to ionization of the imidazolinone ring nitrogen and the phenolic...

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PMID: 10757992
DOI: 10.1021/bi992675o

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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