Article
Effect of additional N-glycosylation signal in the N-terminal region on intracellular function of the human gonadotropin alpha-subunit.
Endocrine journal - 1 Jun 2003
Furuhashi Madoka, Suganuma Nobuhiko
Abstract excerpt
hCG, LH, FSH, and TSH are a family of heterodimeric glycoprotein hormones that contain a common alpha-subunit, but differ in their hormone-specific beta-subunits. The alpha-subunit has two N-glycosylation sites at Asn52 and Asn78. To obtain more information on the relationship between the structure and function of the alpha-subunit, we introduced a novel N-glycosylation site in the N-terminal region by mutating...
Topics
- Amino Acid Sequence
- Animals
- Asparagine
- Aspartic Acid
- CHO Cells
- Chorionic Gonadotropin, beta Subunit, Human
- Cricetinae
- Cricetulus
- Dimerization
- Electrophoretic Mobility Shift Assay
- Glutamine
- Glycoprotein Hormones, alpha Subunit
- Glycosylation
- Humans
- Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
- Mutation
- Terminal Repeat Sequences
- Threonine