Article
Evaluation of a whole-genome amplification method based on adaptor-ligation PCR of randomly sheared genomic DNA.
Genes, chromosomes & cancer - 1 Oct 2003
Tanabe Chikako, Aoyagi Kazuhiko, Sakiyama Tokuki, Kohno Takashi, Yanagitani Noriko, Akimoto Shingo, Sakamoto Michiie, Sakamoto Hiromi, Yokota Jun, Ohki Misao, Terada Masaaki, Yoshida Teruhiko, Sasaki Hiroki
Abstract excerpt
High-throughput genetic studies often require large quantities of DNA for a variety of analyses. Developing and assessing a whole-genome amplification method is thus important, especially with the current desire for large-scale genotyping in previously collected samples for which limited DNA is available. The method we have developed, called PRSG, is based on an adaptor-ligation-mediated PCR of randomly sheared...
Topics
- DNA Ligases
- DNA, Neoplasm
- Deoxyribonuclease BamHI
- Deoxyribonuclease EcoRI
- Deoxyribonucleases, Type II Site-Specific
- Esophageal Neoplasms
- Gene Dosage
- Genome, Human
- Genotype