Role of subunit heteromerization and N-linked glycosylation in the formation of functional hyperpolarization-activated cyclic nucleotide-gated channels.

doi:10.1074/jbc.M306958200

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Article

Role of subunit heteromerization and N-linked glycosylation in the formation of functional hyperpolarization-activated cyclic nucleotide-gated channels.

The Journal of biological chemistry - 31 Oct 2003

Much Barbara, Wahl-Schott Christian, Zong Xiangang, Schneider Angela, Baumann Ludwig, Moosmang Sven, Ludwig Andreas, Biel Martin

Abstract excerpt

The coassembly of homologous subunits to heteromeric complexes serves as an important mechanism in generating ion channel diversity. Here, we have studied heteromerization in the hyperpolarization-activated cyclic nucleotide-gated (HCN) channel family. Using a combination of fluorescence confocal microscopy, coimmunoprecipitation, and electrophysiology we found that upon coexpression in HEK293 cells almost all...

Topics

Animals
Brain
Cell Line
Cell Membrane
Cyclic Nucleotide-Gated Cation Channels
Dimerization
Electrophysiology
Glutamine
Glycoside Hydrolases
Glycosylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels

Immunoblotting

Ion Channels

Mice

Microscopy, Confocal

Microscopy, Fluorescence

Muscle Proteins

Source

Open in PubMed

Identifiers

PMID: 12928435
DOI: 10.1074/jbc.M306958200

Genes

HCN2
HCN3
HCN4
HCN1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-12928435-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Role of subunit heteromerization and N-linked glycosylation in the formation of functional hyperpolarization-activated cyclic nucleotide-gated channels.PMID 12928435Read ↗

Effects of N-glycosylation on hyperpolarization-activated cyclic nucleotide-gated (HCN) channels.Semantic proximity Read ↗

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