Article
Adiponectin gene activation by thiazolidinediones requires PPAR gamma 2, but not C/EBP alpha-evidence for differential regulation of the aP2 and adiponectin genes.
Biochemical and biophysical research communications - 5 Sept 2003
Gustafson Birgit, Jack Maia M, Cushman Samuel W, Smith Ulf
Abstract excerpt
We examined the role of PPAR gamma 2 and C/EBP alpha for adiponectin and aP2 gene activation in C/EBP alpha(-/-) fibroblasts by stably expressing PPAR gamma 2 or C/EBP alpha. PPAR gamma 2, but not PPAR gamma 1, mRNA markedly increased during the differentiation to adipocytes in cells expressing C/EBP alpha. Both infected cell lines differentiated to an adipocyte phenotype and the mRNA for both aP2 and adiponectin...
Topics
- 3T3 Cells
- Adipocytes
- Adiponectin
- Animals
- Anticonvulsants
- CCAAT-Enhancer-Binding Protein-alpha
- Cell Differentiation
- Cell Line
- Culture Media
- Cytokines
- Fibroblasts
- Fructose
- Gene Expression Regulation