Article
Expression and transmission of wild-type pigmentation in the skin of transgenic orange-colored variants of medaka (Oryzias latipes) bearing the gene for mouse tyrosinase.
Pigment cell research - 1 Nov 1992
Matsumoto J, Akiyama T, Hirose E, Nakamura M, Yamamoto H, Takeuchi T
Abstract excerpt
Transgenic fish carrying a reconstructed mouse tyrosinase gene, mg-Tyrs-J, were produced by microinjecting the gene into the oocyte nucleus of an orange-colored variant of medaka (Oryzias latipes). Of 64 oocytes microinjected and subsequently inseminated, 13 embryos developed normally beyond hatching and three of them exhibited brown skin pigmentation in the adult as was commonly observed in the wild type of this...
Topics
- Animals
- Animals, Genetically Modified
- Female
- Fluorescent Antibody Technique
- Gene Expression Regulation, Enzymologic
- Genetic Variation
- Immunohistochemistry
- Male
- Melanocytes
- Melanophores