Article
Molecular handcuffing of the relaxosome at the origin of conjugative transfer of the plasmid R1162.
Nucleic acids research - 15 Aug 2003
Zhang Xiaolin, Zhang Shuyu, Meyer Richard J
Abstract excerpt
The assembly of plasmid-encoded proteins at a unique site (oriT) on the plasmid R1162, to form a complex called the relaxosome, is required for conjugative transfer of the plasmid and for negative regulation of neighboring promoters. Two-dimensional chloroquine gel electrophoresis was used to show that oriTs are physically coupled at the relaxosome. This interaction requires all the relaxosome proteins, which are...
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