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Article

Construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains.

Protein engineering - 1 Dec 1992

Iwakura M, Matthews C R

Abstract excerpt

A single polypeptide chain containing two dihydrofolate reductase (DHFR) sequences from Escherichia coli was constructed to determine if a repeat sequence fusion protein could be expressed in an active form. The possibility that intersequence interactions could play a significant role for this enzyme is suggested by the results of Hall and Frieden (1989, Proc. Natl Acad. Sci. USA, 86, 3060-3064) who observed a...

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Identifiers

PMID: 1287660
DOI: 10.1093/protein/5.8.791

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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