Effects of the length of a glycine linker connecting the N-and C-termini of a circularly permuted dihydrofolate reductase.

doi:10.1093/protein/11.8.707

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Article

Effects of the length of a glycine linker connecting the N-and C-termini of a circularly permuted dihydrofolate reductase.

Protein engineering - 1 Aug 1998

Iwakura M, Nakamura T

Abstract excerpt

An important consideration in the construction of active and stable circularly permuted proteins is the connective sequence that links the native N- and C-termini. For this reason, various lengths of polyglycine linkers (two, three, four, five and six glycines) were employed to connect the origin...

Topics

Base Sequence
Chromatography, Gel
Circular Dichroism
Enzyme Stability
Glycine
Leucine
Methionine
Molecular Sequence Data
Mutation
Protein Biosynthesis
Protein Conformation
Protein Denaturation
Recombinant Proteins
Tetrahydrofolate Dehydrogenase
Urea

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