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Article

Substrate binding and catalysis in trichosanthin occur in different sites as revealed by the complex structures of several E85 mutants.

Protein engineering - 1 Jun 2003

Guo Qing, Zhou Weihong, Too Hiu-Mei, Li Jianhui, Liu Yiwei, Bartlam Mark, Dong Yicheng, Wong Kam-Bo, Shaw Pang-Chui, Rao Zihe

Abstract excerpt

Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) which possesses rRNA N-glycosidase activity. In recent years, its immunomodulatory, anti-tumor and anti-HIV properties have been revealed. Here we report the crystal structures of several E85 mutant TCS complexes with adenosine-5'-monophosphate (AMP) and adenine. In E85Q TCS/AMP and E85A TCS/AMP, near the active site of the molecule and parallel...

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Identifiers

PMID: 12874371
DOI: 10.1093/protein/gzg056

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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