Article
Role of Arg163 in the N-glycosidase activity of neo-trichosanthin.
Protein engineering - 1 Nov 1999
Li H G, Xu S Z, Wu S, Yan L, Li J H, Wong R N, Shi Q L, Dong Y C
Abstract excerpt
Three mutant crystals of neo-trichosanthin (n-TCS), R163K, R163H and R163Q, were obtained by the hanging drop vapor diffusion method. Structure determination indicated that there are no significant differences between the mutants and n-TCS except in the active pocket. All of them were also soaked in sodium citrate buffer (pH 4. 5) containing 20% KCl and 10 mg/ml AMP. Structure determination suggests that in the...
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