Article
Crystal structure of the Tyr45Trp mutant of ribonuclease T1 in a complex with 2'-adenylic acid.
European journal of biochemistry - 1 Oct 1991
Koellner G, Grunert H P, Landt O, Saenger W
Abstract excerpt
The recombinant Tyr45Trp mutant of Lys25-ribonuclease T1 was overexpressed and purified from an Escherichia coli strain. The mutant enzyme, which shows reduced activity towards GpA and increased activity towards pGpC, pApC and pUpC compared with wild-type RNase T1, was co-crystallized with 2'-adenylic acid by microdialysis. The space group is P212121 with unit cell dimenions a = 4.932(2), b = 4.661(2), c =...
Topics
- Adenosine Monophosphate
- Binding Sites
- Crystallization
- Escherichia coli
- Guanine
- Molecular Structure
- Mutation
- Protein Conformation
- Recombinant Proteins
- Ribonuclease T1
- Substrate Specificity