Article
The antigenic sites of trichosanthin, a ribosome-inactivating protein with multiple pharmacological properties.
Life sciences - 1 Jan 1997
Mulot S, Chung K K, Li X B, Wong C C, Ng T B, Shaw P C
Abstract excerpt
Trichosanthin (TCS) fragments were produced by Tn1000 deletion mutagenesis and by cyanogen bromide (CNBr) cleavage and their immunoreactivity was examined by incubating with various antibodies. Twelve C-terminally truncated TCS variants were successfully synthesized under the control of a T7 RNA driven promoter. The smallest antigenic fragment mapped corresponded to the N-terminal 20 amino acids (aa). Six CNBr...
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