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Article

Heteroduplex-based genotyping with microchip electrophoresis and dHPLC.

Genetic testing - 1 Jan 2003

Footz Tim, Somerville Martin J, Tomaszewski Robert, Sprysak Kathleen A, Backhouse Christopher J

Abstract excerpt

This work compares the methods of mutation detection via denaturing high-performance liquid chromatography (dHPLC) and a microchip-based heteroduplex analysis (HA) method. The mutations analyzed were 185delAG and 5382insC in BRCA1 and 6174delT in BRCA2 with, as additional examples, 188del11 and 5396 + 1G --> A in BRCA1. Our HA method is based upon the use of a replaceable, highly denaturing sieving matrix that...

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Share to BRCA1 Protein
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Share to Chromatography, High Pressure Liquid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Mutational Analysis

Share to Electrophoresis

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Share to Oligonucleotide Array Sequence Analysis

Share to Sensitivity and Specificity

Source

Identifiers

PMID: 15000804
DOI: 10.1089/109065703322783635

Genes

BRCA1
BRCA2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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