Article
The contribution of polar group burial to protein stability is strongly context-dependent.
The Journal of biological chemistry - 22 Aug 2003
Takano Kazufumi, Scholtz J Martin, Sacchettini James C, Pace C Nick
Abstract excerpt
We previously suggested that proteins gain more stability from the burial and hydrogen bonding of polar groups than from the burial of nonpolar groups (Pace, C. N. (2001) Biochemistry 40, 310-313). To study this further, we prepared eight Thr-to-Val mutants of RNase Sa, four in which the Thr side chain is hydrogen-bonded and four in which it is not. We measured the stability of these mutants by analyzing their...
Topics
- Crystallography, X-Ray
- Mutation
- Protein Conformation
- Protein Denaturation
- Proteins