Open complex formation in vitro by sigma38 (rpoS) RNA polymerase: roles for region 2 amino acids.

Abstract excerpt

Non-functional mutants of sigma(38)(sigma(S)) were studied in vitro to identify the nature of their defects. Mutations in four amino acids led to severe defects in DNA binding and enzyme isomerization with promoter fork junction probes containing single-stranded non-template DNA. The same properties were previously seen with DNA mutations at the fork junction, implying that sigma:DNA interactions at the fork...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Amino Acids
• Share to DNA Probes
• Share to DNA, Single-Stranded