Article
Open complex formation in vitro by sigma38 (rpoS) RNA polymerase: roles for region 2 amino acids.
Journal of molecular biology - 20 Jun 2003
Lee Shun Jin, Gralla Jay D
Abstract excerpt
Non-functional mutants of sigma(38)(sigma(S)) were studied in vitro to identify the nature of their defects. Mutations in four amino acids led to severe defects in DNA binding and enzyme isomerization with promoter fork junction probes containing single-stranded non-template DNA. The same propert...
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