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Article

In vitro roles of invariant helix-turn-helix motif residue R383 in sigma(54) (sigma(N)).

Nucleic acids research - 1 Mar 2001

Wigneshweraraj S R, Ishihama A, Buck M

Abstract excerpt

In vitro DNA-binding and transcription properties of sigma(54) proteins with the invariant Arg383 in the putative helix-turn-helix motif of the DNA-binding domain substituted by lysine or alanine are described. We show that R383 contributes to maintaining stable holoenzyme-promoter complexes in which limited DNA opening downstream of the -12 GC element has occurred. Unlike wild-type sigma(54), holoenzymes...

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Identifiers

PMID: 11222766
DOI: 10.1093/nar/29.5.1163
PMCID: PMC29711

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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