Article
Changing patterns of gene expression in dictyostelium prestalk cell subtypes recognized by in situ hybridization with genes from microarray analyses.
Eukaryotic cell - 1 Jun 2003
Maeda Mineko, Sakamoto Haruyo, Iranfar Negin, Fuller Danny, Maruo Toshinari, Ogihara Satoshi, Morio Takahiro, Urushihara Hideko, Tanaka Yoshimasa, Loomis William F
Abstract excerpt
We used microarrays carrying most of the genes that are developmentally regulated in Dictyostelium to discover those that are preferentially expressed in prestalk cells. Prestalk cells are localized at the front of slugs and play crucial roles in morphogenesis and slug migration. Using whole-mount in situ hybridization, we were able to verify 104 prestalk genes. Three of these were found to be expressed only in...
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