Article
Subsite mapping of human salivary alpha-amylase and the mutant Y151M.
FEBS letters - 5 Jun 2003
Kandra Lili, Gyémánt Gyöngyi, Remenyik Judit, Ragunath Chandran, Ramasubbu Narayanan
Abstract excerpt
This study characterizes the substrate-binding sites of human salivary alpha-amylase (HSA) and its Y151M mutant. It describes the first subsite maps, namely, the number of subsites, the position of cleavage sites and apparent subsite energies. The product pattern and cleavage frequencies were determined by high-performance liquid chromatography, utilizing a homologous series of chromophore-substituted...
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