A mutant alpha-amylase with enhanced activity specific for short substrates.

doi:10.1016/0014-5793(92)81335-j

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Article

A mutant alpha-amylase with enhanced activity specific for short substrates.

FEBS letters - 5 Oct 1992

Matsui I, Ishikawa K, Miyairi S, Fukui S, Honda K

Abstract excerpt

The 210th lysine (K210) at the active site in Saccharomycopsis fibuligera alpha-amylase was altered to arginine (R) or asparagine (N) by site-directed mutagenesis. Replacement of K210 by R strengthened the 7th and weakened the 8th subsite affinities. K210 was found to contribute to both the 8th a...

Topics

Amylose
Binding Sites
Maltose
Mutagenesis, Site-Directed
Mutation
Oligosaccharides
Saccharomycopsis
Substrate Specificity
alpha-Amylases

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