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Article

Crystal structures of the ribonuclease MC1 mutants N71T and N71S in complex with 5'-GMP: structural basis for alterations in substrate specificity.

Biochemistry - 13 May 2003

Numata Tomoyuki, Suzuki Akio, Kakuta Yoshimitsu, Kimura Kazumi, Yao Min, Tanaka Isao, Yoshida Yuichiro, Ueda Tadashi, Kimura Makoto

Abstract excerpt

Ribonuclease MC1 (RNase MC1), isolated from bitter gourd seeds, is a uridine specific RNase belonging to the RNase T2 family. Mutations of Asn71 in RNase MC1 to the amino acids Thr (N71T) and Ser (N71S) in guanosine preferential RNases altered the substrate specificity from uridine specific to guanosine specific, as shown by the transphosphorylation of diribonucleoside monophosphates [Numata, T., et al. (2001)...

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PMID: 12731868
DOI: 10.1021/bi034103g

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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