Article
Non-cognizable ribonucleotide, 2'AMP, binds to a mutant ribonuclease T1 (Y45W) at a new base-binding site but not at the guanine-recognition site.
FEBS letters - 23 Sept 1991
Hakoshima T, Itoh T, Gohda K, Tomita K, Uesugi S, Nishikawa S, Morioka H, Ohtsuka E, Ikehara M
Abstract excerpt
Complex of a mutant ribonuclease T1 (Y45W) with a non-cognizable ribonucleotide, 2'AMP, has been determined and refined by X-ray diffraction at 1.7 A resolution. The 2'AMP molecule locates at a new base-binding site which is remote from the guanine-recognition site, where 2'GMP was found to be bound. The nucleotide adopts the anti conformation of the glycosidic bond and C3'-exo sugar pucker. There exists a single...
Topics
- Adenosine Monophosphate
- Amino Acid Sequence
- Aspergillus oryzae
- Binding Sites
- Crystallography
- Guanosine Monophosphate
- Hydrogen Bonding
- Isomerism
- Models, Molecular
- Molecular Structure