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Article

Conformation of 2'GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR methods.

Journal of biochemistry - 1 Nov 1991

Itoh T, Tomita K, Hakoshima T, Hiroaki H, Uesugi S, Nishikawa S, Amisaki T, Morioka H, Ohtsuka E, Ikehara M

Abstract excerpt

The crystal structure of a mutant ribonuclease T1 (Y45W) complexed with a specific inhibitor, 2'GMP, has been determined by X-ray diffraction and refined at 1.9 A resolution to a conventional R-factor of 0.164. The mode of recognition of the guanine base by the enzyme is similar to that found for...

Topics

Carbohydrate Conformation
Escherichia coli
Guanosine Monophosphate
Hydrogen Bonding
Magnetic Resonance Spectroscopy
Mutation
Protein Binding
Protein Conformation
Ribonuclease T1
Solutions
Stereoisomerism

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

X-Ray Diffraction

Source

Identifiers

PMID: 1664424
DOI: 10.1093/oxfordjournals.jbchem.a123639

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1664424-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Conformation of 2'GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR methods.PMID 1664424Read ↗

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