Article
Absence of exon 10 of the human luteinizing hormone (LH) receptor impairs LH, but not human chorionic gonadotropin action.
The Journal of clinical endocrinology and metabolism - 1 May 2003
Müller Thomas, Gromoll Jörg, Simoni Manuela
Abstract excerpt
The LH receptor (LHR) mediates the actions of LH and human chorionic gonadotropin (hCG). In vivo data showed that deletion of exon 10 does not affect hCG action, whereas LH action is impaired. To investigate the role of exon 10 in LH/hCG action in vitro we created stable COS-7 cells expressing the LHR with (wt) or without (-ex10) exon 10. Binding experiments showed that the affinities of LH and hCG to the LHR wt...
Topics
- Animals
- COS Cells
- Chorionic Gonadotropin
- Cyclic AMP
- Exons
- Humans
- Iodine Radioisotopes
- Luteinizing Hormone
- Mutation
- Pertussis Toxin
- Protein Conformation
- Receptors, LH
- Signal Transduction
- Structure-Activity Relationship
- Transfection