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Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures.

Nucleic acids research - 15 Apr 2003

Droogmans Louis, Roovers Martine, Bujnicki Janusz M, Tricot Catherine, Hartsch Thomas, Stalon Victor, Grosjean Henri

Abstract excerpt

N1-methyladenosine (m1A) is found at position 58 in the T-loop of many tRNAs. In yeast, the formation of this modified nucleoside is catalyzed by the essential tRNA (m1A58) methyltransferase, a tetrameric enzyme that is composed of two types of subunits (Gcd14p and Gcd10p). In this report we describe the cloning, expression and characterization of a Gcd14p homolog from the hyperthermophilic bacterium Thermus...

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PMID: 12682365
DOI: 10.1093/nar/gkg314
PMCID: PMC153742

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