Article
Absence of malolactic activity is a characteristic of H+-ATPase-deficient mutants of the lactic acid bacterium Oenococcus oeni.
Applied and environmental microbiology - 1 Apr 2003
Galland Delphine, Tourdot-Maréchal Raphaëlle, Abraham Maud, Chu Ky Son, Guzzo Jean
Abstract excerpt
The lack of malolactic activity in H(+)-ATPase-deficient mutants of Oenococcus oeni selected previously was analyzed at the molecular level. Western blot experiments revealed a spot at 60 kDa corresponding to the malolactic enzyme only in the parental strain. Moreover, the mleA transcript encoding the malolactic enzyme was not detected by reverse transcription (RT)-PCR analysis of mutants. These results suggest...
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