Article
Absence of malolactic activity is a characteristic of H+-ATPase-deficient mutants of the lactic acid bacterium Oenococcus oeni.
Applied and environmental microbiology - 1 Apr 2003
Galland Delphine, Tourdot-Maréchal Raphaëlle, Abraham Maud, Chu Ky Son, Guzzo Jean
Abstract excerpt
The lack of malolactic activity in H(+)-ATPase-deficient mutants of Oenococcus oeni selected previously was analyzed at the molecular level. Western blot experiments revealed a spot at 60 kDa corresponding to the malolactic enzyme only in the parental strain. Moreover, the mleA transcript encoding the malolactic enzyme was not detected by reverse transcription (RT)-PCR analysis of mutants. These results suggest...
Topics
- Gene Deletion
- Gene Expression Regulation, Bacterial
- Gram-Positive Cocci
- Immunoblotting
- Lactic Acid
- Lactococcus lactis
- Leuconostoc
- Malate Dehydrogenase
- Malates
- Mutation
- Proton-Translocating ATPases
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription, Genetic