Article
[Isolation, characterization and real-time RT-PCR for relative quantification of gene expression in H+-ATPase-defective mutants from Lactobacillus plantarum].
Wei sheng wu xue bao = Acta microbiologica Sinica - 4 Feb 2017
Zhang Xiang, Fang Hui, Xie Dongfang, Lin Yuecheng, Tao Yingyan, Wang Hongpeng, Gong Jinyan, Ge Qing, Pan Guorong, Huang Jun, You Yuru
Abstract excerpt
Objective: The aim of this study was to isolate Lactobacillus plantarum acid-sensitive mutants with lower H+-ATPase activity, and to study the mechanism of H+-ATPase regulation in Lactobacillus plantarum. Methods: We used neomycin to isolate acid-sensitive mutants of L. plantarum, and measured H+-ATPase activity and lactic acid production of wild-type and mutants. Genomic DNA was extracted from the wild-type ZUST...
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