Article
Characterization of a mutant of Lactococcus lactis with reduced membrane-bound ATPase activity under acidic conditions.
Bioscience, biotechnology, and biochemistry - 1 Aug 1998
Amachi S, Ishikawa K, Toyoda S, Kagawa Y, Yokota A, Tomita F
Abstract excerpt
A mutant of Lactococcus lactis subsp. lactis C2 with reduced membrane-bound ATPase activity was characterized to clarify its acid sensitivity. The cytoplasmic pH of the mutant was measured in reference to the parental strain under various pH conditions. At low pH, the mutant could not maintain it...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Blotting, Western
- Cell Membrane
- Cytoplasm
- DNA Probes
- DNA, Bacterial
- Electrophoresis, Agar Gel
- Electrophoresis, Polyacrylamide Gel
- Fermentation
- Gene Expression Regulation, Bacterial
- Hydrogen-Ion Concentration
- Lactococcus lactis
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Proton-Translocating ATPases