Article
Reagentless optical sensing of glutamine using a dual-emitting glutamine-binding protein.
Analytical biochemistry - 15 Mar 2003
Tolosa Leah, Ge Xudong, Rao Govind
Abstract excerpt
Glutamine is a major source of nitrogen and carbon in cell culture media. Thus, glutamine monitoring is important in bioprocess control. Here we report a reagentless fluorescence sensing for glutamine based on the Escherichia coli glutamine-binding protein (GlnBP) that is sensitive in the submicromolar ranges. The S179C variant of GlnBP was labeled at the -SH and N-terminal positions with acrylodan and ruthenium...
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