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Article

Engineering a switch-based biosensor for arginine using a Thermotoga maritima periplasmic binding protein.

Analytical biochemistry - 15 May 2017

Donaldson Teraya, Iozzino Luisa, Deacon Lindsay J, Billones Hilbert, Ausili Alessio, D'Auria Sabato, Dattelbaum Jonathan D

Abstract excerpt

The Thermotoga maritima arginine-binding protein (TmArgBP) has been modified to create a reagentless fluorescent protein biosensor. Two design methods for biosensor construction are compared: 1) solvent accessibility of environmentally-sensitive probes and 2) fluorescence deactivation due to photo-induced electron transfer (PET). Nine single cysteine TmArgBP mutants were created and labeled with three different...

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Identifiers

PMID: 28259516
DOI: 10.1016/j.ab.2017.02.021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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